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产品展示大星彩票注册 > 产品展示 > > 蛋白交互作用分析系统 > 蛋白交互作用分析系统

产品名称:蛋白交互作用分析系统

产品型号:

产品特点:蛋白交互作用分析系统主要用于生物学领域的冷光和荧光检测波长测定:如
FRET(荧光共振能量转移)分子设计的调查
BRET(生物发光共振能量转移)分子设计的调查
荧光/冷光试剂的发展和质量控制
荧光/冷光反应的分析

蛋白交互作用分析系统的详细资料:

蛋白交互作用分析系统

PINPAIJIESHAO:

ATTOZHUSHIHUISHECHENGLIYU1964NIAN,SHIRIBENzuiDA、SHIJIEzuiZHUMINGDEDIANYONGCHANGSHANGZHIYI,JUYOUWANZHENGDECHANGGUIDIANYONGCHANPINXILIE。ZUOWEIRIBEN*DEDIANYONGPINPAI,ATTOYIZHIBINGCHENGZHE“SHIJIEXINLAIDEPINZHI”DEJINGSHEN,QICHANPINPINGJIEWUYULUNBIDEJINGZHIHEKEKAODEXINGNENG,JUYOULECHAOYUEQITAPINPAICHANPINDESHILI,CHENGWEIXIAOXINGDIANYONGCHANPINDELINGHANGZHE。

大星彩票注册ATTOZHUSHIHUISHETONGYANGYESHISHIJIESHANGzuiZAOYANZHIYINGGUANGFAGUANGYIQIDECHANGJIAZHIYI。SUIZHERENLEIGENGSHENCENGCIDETANSUOSHENGMINGDEAOMI,YINGGUANGJIANCEYICHENGWEIYIZHONGFEICHANGYOUXIAODESHOUDUAN。ATTOYITUOXIANJINDEJISHUHEQIANGDADEKEYANSHILI,LIANXUKAIFACHUDUOKUANGAODUANDEYINGGUANGJIANCEYIQI,ZAIYINGYONGSHANGTUPOLECHUANTONGYINGGUANGYIQIDEJUXIANXING,BAZHEIXIANGJISHUYOUTIGAODAOYIGEXINDESHUIPING,MANZUGENGGAOYAOQIUDEKEYANGONGZUOYAOQIU。

蛋白交互作用分析系统

CHANPINXINGNENG:

主要用于生物学领域的冷光和荧光检测波长测定:如

大星彩票注册FRET(YINGGUANGGONGZHENNENGLIANGZHUANYI)FENZISHEJIDEDIAOCHA

大星彩票注册BRET(SHENGWUFAGUANGGONGZHENNENGLIANGZHUANYI)FENZISHEJIDEDIAOCHA

大星彩票注册YINGGUANG/LENGGUANGSHIJIDEFAZHANHEZHILIANGKONGZHI

YINGGUANG/LENGGUANGFANYINGDEFENXI

 

用途特征;

大星彩票注册1. FENGUANGGONGNENG;TONGSHICEDINGXIANGTONGTIAOJIANXIADECELIANGFANWEINEIBUTONGBOZHANGDEGUANG

2. YIGELENGCCDXIANGJIZUOWEIJIANCEQI,TONGGUODIAOJIEPUGUANGSHIJIANJIKEZENGJIAJIANCELINGMINDU

LumiFL-Spectro capture用一个高灵敏度的冷CCD做检测器,增加了灵敏度,同时有利于整个波长范围内的同时测量。这个单元是由光分离部分和检测装置做为基本结构;同时,如果客户需要测量的样品是靠荧光激发的,还能连接荧光激发光源进行测定(可选:请在要求中注明)。荧光样品的测定可以用石英材质的容器,冷光样品可以用塑料的小离心管。

 

硬件特征:

1、DIANZIDELENGCCDXIANGJIZUOWEIJIANCEQI

2、CCDKEYITONGSHIJIESHOUHEJIANCEZHENGGEMUBIAOBOZHANGFANWEIQUDESUOYOUGUANG

3、CCDBIGUANGDIANBEIZENGGUANPMTYOUGENGGAODELIANGZIXIAOLV

4、SUOYOUBOZHANGTONGSHIJIANCE,DADASUODUANLEJIANCESHIJIAN

5、CEDINGBOZHANGFANWEI:400-800nm

6、KEYIZHANGSHIJIANPUGUANG(TIGAOLEWEIXIAOYANGPINDEJIANCELINGMINDU)

大星彩票注册7、RUGUOZHUANGZHIYINGGUANGJIFAGUANGYUAN,HAIKEYICEDINGFRETHUOYINGGUANGDIWU

 

与常规的分光计比较如下:

大星彩票注册YAOBUHUORUOGUANGBOZHANGNEIDEQUANBUBIANHUA,BIXUYAOYOUCEDINGXIAOYANGPINDEGAOLINGMINDUDEJIANCEZHUANGZHI。CHANGGUIDEGUANGDUJISHIZAIBAGUANGFENSANHOUCAIYONGGUANGDIANBEIZENGGUAN(PMT)YIGEGEDEJIANCE,ZHEIYANGDEHUA,MINGANDUDADAJIANGDI,TONGSHIKENENGHUIYOUYUYIXIAYUANYINMEIYOUDADAOYIDINGGUANGMIDU,DAOZHIYANGPINBUNENGCEDINGCHUGUANGPU:

(1)PMTDEDINGLIANGXIAOLVBENSHENJIUBICCDDI

(2)CEDINGSHIJIANDUAN

GENGZHONGYAODESHI,RUGUOWEIBUCHANGDILINGMINDUDEBUBIANERJIANGSAOMIAOSHIJIANYANZHANG,LENGGUANGHUOYINGGUANGJIUHUIZHUJIANBIANXI,FANERDEBUCHANGSHI,DEBUDAOJINGQUEDEGUANGPU。

 

LumiFL-Spectro capture

CHANGGUIGUANGDUJI

JIANCEQI

LENGDECCD

PMT

JIANCEFANGSHI

CHENGPILIANG

YIGEYIGEDI、SHUNXUCEDING

SHIJIAN

DUAN

ZHANG

MUBIAO

GUANGQIANGDIDEGUANG

GAOQIANGDUGUANG

 

样品测量:

发光样品——滤光片元件组——细缝元件组大星彩票注册——衍射光谱——所有波长的光通过冷CCD成批量检测

1.YANGPINKEYIZAISHIYINGBEIHUOZHEPCRGUANNEICEDING;LENGGUANGYANGPIN(XIBAOHUODANBAI)DOUKEYICE

大星彩票注册2.JUGUANGJING/LVGUANGPIAN:KEYISHEDINGDANPIANJIFAGUANGLVPIAN

大星彩票注册3.XIFENGYUANJIANZU:KEYIJUJIYANGPINGUANG

大星彩票注册4.YANSHEGUANGZHA:FENLIYANGPINGUANG

大星彩票注册5.JIANCEYUANJIAN:ZHENGGEYANGPINDEGUANGBEIFENLIHOUDEGUANGPUKEJINGLENGCCDTONGSHICEDING;CELIANGSHIJIANDUAN,KEYITONGGUOZHANGSHIJIANPUGUANGDADAOTIGAOLINGMINDUDEXIAOGUO。

 

举例:

BRETYANGPINDECEDING:

当虫荧光素加到蓝色荧光素酶Vluc和黄色荧光蛋白YFP的融合蛋白上,荧光素和酶反应产生能量。一部分能量转移到YFP上,BRET反应就开始了。如果我们插入一小段包含目的序列(KR)的短肽片段,它可以转运酶PC1到Vluc-PC1这个复合蛋白上,识别并优先结合YFP的肽链序列。用AB-1850测定这个融合物的光谱,460nm下的Vluc冷光和527nm下的YFP都发出了强光,这样BRET反应被有力地证实了。接下来,PC1加入反应体系,527nm下的光减弱了,峰值变小。这个结果可以这样解释:PC1抢先结合到复合蛋白上,切断了能量转移到GFP上的通路,所以BRET的效率降低,通过检测数量的降低可以检测PC1的活性。

生物技术领域的用途:

BRET大星彩票注册或FRET用途中的评估分子设计

大星彩票注册WEIWANCHENGBRETHUOFRETDESHIYAN,SHOUXIANXUYAOYUSHIYANJIEDUANDEJIANYANFENZISHEJI。ZHEIZHONGQINGKUANGXIA,YONGGUANGXUELVGUANGPIANJIANCELENGGUANGHUOYINGGUANGYANGPINBUKENENGBAYANGPINDEQUANBUFAGUANGBOZHANGDOUJIANCEDAO,TONGSHIYEBUNENGGUOYOUXIAOERCHONGFENDIJIANCEDAONENGLIANGZHUANYIQIJIANBOZHANGDEBIANHUA。ERRUGUOYONGAB-1850,ZHEIXIEJIUBUCHENGWENTILE,KEYIYOUXIAODIJINXINGFENZISHEJI。

BRETHUOFRET:zuiJINZHEILIANGZHONGJISHUCHENGWEIREMEN,BEIGUANGFANYINGYONGYUFENXIDANBAIJIAN、DANBAIHEHESUANJIANDE,HUOZHEDUIDANBAIJINXINGXIUSHI、JIANQIEHEKONGJIANGOUXIANGGAIBIANHOUDEGONGNENGYANJIU。

FRETSHIYIZHONGXIANXIANG,CONGJIFAYINGGUANGDEFENZI(GONGTI)CHANSHENGDEGUANGNENGLIANGJIHUOLEQITAYINGGUANGFENZI(SHOUTI),CONGERSHOUTIFAGUANG。TAYIBANFASHENGZAISHOUTIHEGONGTIJIANCUNZAIYIDINGDEJULIHEKONGJIANGUANXI,TONGGUOCEDINGFACHUDEGUANGDEBOZHANG,JIUKEYIPANDUANFRETSHIFOUFASHENG。

BRET 中大星彩票注册,荧光分子(供体)被冷光分子所取代,光也不是由供体发出,而是受体被冷光分子的冷光激活。BRET 中,如果冷光底物在测定时加入,检测就可以进行了,所以不需要高能量的激发光源装置。这样就降低了对样品的损伤。

XINGNENGCANSHU:

大星彩票注册1.LENGGUANG、YINGGUANGCEDINGZHUANGZHI

 

 F值 F3.0
 测量波长范围 400-800nm
 逆线性色散 60nm/mm
 波长分辨率 1.8nm
 重复性 ±0.6nm
 隙缝宽度 0.01nm-5nm(11步)
 曝光时间 1/20s----60min(26步)
 光接收装置 电子冷CCD

 

2.GUANGPUCHENGXIANG:

YANSHEGUANGZHASHUMU:1GE(BIAOZHUN)GUANGZHA:150pc/mm

Braze BOZHANG:500nm

3.CELIANGYANGPINBUFEN:

CEDINGQIMIN:SHIYINGBEI(4mm*4mm)/ 0.2ml PCRGUAN/ 35mm PEIYANGMIN

大星彩票注册YANGPINTIJI:10ul-100ul

SHUNSHILENGGUANGCEDING:ZHURUKONG(GUITONGGEBAN)

大星彩票注册JIFAGUANGYUANDELIANJIE:FCJIEGUAN(BIAOPEI)

4.RUANJIAN:

GONGNENG:CELIANGHEGUANGBOSHUJUFENXI

JIEKOU: USB2.0

5.TIJIHEZHONGLIANG:

DAXIAO:510mm(W)*725mm(D)*390mm(H)

大星彩票注册ZHONGLIANG:58kg            

DIANYUAN:C100v  50/60Hz  290V

6.BIAOZHUNPEIJIAN:

大星彩票注册LumiFL-Spectro GUANGBUHUOXITONG:1TAI

35mm PINGMINSHIYANZUQU:1PC

大星彩票注册FANGXINGXIBAOYANGPINQU:1PC

PCRGUANYANGPINQU:1

YINGGUANGCELIANGSHIDELVJINGTUOBAN:1

ACSHENG:1

USBJIEXIAN:1

SHUNSHIYINGGUANGCEDINGSHIXUYAOYONGDAODESHIJIJIAYANGJIEGUAN:1

SHIYINGRONGQI:1

PCRGUAN:1DAI

WEISHUNSHICEDINGTIGONGDESHIJIJIAYANGXIANGJIAOFA:1DAI

SHUJUFENXIRUANJIAN(CDBAN):1

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